Coconut milk ameliorates motor and non-motor deficits in the alpha-synuclein transgenic Drosophila melanogaster model for Parkinsonism


  • Olumayowa Idowu Osun State University, Osogbo
  • Olufunke Dosumu
  • Ademola Oremosu


Alpha-synuclein, Coconut milk, Drosophila melanogaster, Fecundity, Non-motor dysfunction, Parkinson’s disease


Context: Parkinson's disease (PD) is a multisystem neurodegenerative disorder associated with oxidative stress and disrupted mitochondrial function. It is characterized by motor and non-motor symptoms due to multi-factors. Coconut (Cocos nucifera) milk contains a complex mixture of highly nutritional constituents such as carbohydrates, vitamins and minerals which have remarkable health benefits. Lauric acid (LA) which makes up to 54.5% of total nutrient composition of coconut milk (CM) possesses strong antioxidant properties. Objectives: This study evaluated the effect of CM on PD-associated motor and non-motor deficits in alpha-synuclein (α-syn) transgenic Drosophila melanogaster (D. melanogaster). Methods: W1118 strain (control) and α-syn transgenic (PD strain) of D. melanogaster aged between 1 to 3 days were randomized into various groups. The control flies were fed on untreated diets, while the PD flies were orally exposed to varied concentrations (0, 5, 10 and 15% v/v) of coconut milk diet for 14 days. Subsequently, longevity, behavioral, fecundity and biochemical analyses were conducted across the groups. Results: The results showed that CM significantly increased longevity, climbing ability, egg count and the rate of emergence (P< 0.05). In addition, MDA and NO levels as well as AChE activity were significantly decreased, while GSH level alongside SOD and CAT activities were increased (P< 0.05) in the CM-treated PD flies in a concentration-dependent manner. Conclusions: CM ameliorated PD-associated deficits in D. melanogaster by prolonging lifespan, improving locomotor function, fecundity and redox status.


Abolaji, A. O., Adedara, A. O., Adie, M. A., Vicente-Crespo, M., & Farombi, E. O. (2018). Resveratrol prolongs lifespan and improves 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine-induced oxidative damage and behavioural deficits in Drosophila melanogaster. Biochemical and biophysical research communications, 503(2), 1042-1048.

Aebi, H. (1984). Catalase in vitro. Methods in Enzymology 105, 121-126.

Alabi, A. O., Ajayi, A. M., Ben-Azu, B., Bakre, A. G., & Umukoro, S. (2019). Methyl jasmonate abrogates rotenone-induced parkinsonian-like symptoms through inhibition of oxidative stress, the release of pro-inflammatory cytokines, and down-regulation of immunopositive cells of NF-κB and α-synuclein expressions in mice. Neurotoxicology, 74, 172-183.

Bendor, J. T., Logan, T. P., & Edwards, R. H. (2013). The function of α-synuclein. Neuron, 79(6), 1044-1066.

Benjamin, A., Charity, O.N., Peter, A., & Miebaka, O. (2017). Nutrient composition and ameliorative effects of Cocos nucifera products on Alloxan-induced diabetic Wistar rats. International Journal of Medicine, 5(2).

Boemeke, L., Marcadenti, A., Busnello, F. M., & Gottschall, C. B. (2015). Effects of coconut oil on human health. Open Journal of Endocrine and Metabolic Diseases, 5(07), 84.

Casertano, M., Fogliano, V., & Ercolini, D. (2021). Psychobiotics, gut microbiota, and fermented foods can help preserve mental health. Food Research International, 110892.

Cunnane, S. C., Courchesne‐Loyer, A., St‐Pierre, V., Vandenberghe, C., Pierotti, T., Fortier, M., . . . Castellano, C. (2016). Can ketones compensate for deteriorating brain glucose uptake during aging? Implications for the risk and treatment of Alzheimer's disease. Annals of the New York Academy of Sciences, 1367(1), 12-20.

D'Souza, L. C., Dwivedi, S., Raihan, F., Yathisha, U. G., Raghu, S. V., Mamatha, B. S., & Sharma, A. (2022). Hsp70 overexpression in Drosophila hemocytes attenuates benzene‐induced immune and developmental toxicity via regulating ROS/JNK signaling pathway. Environmental toxicology.

Degger, N., Anna, C., & Wu, R. S. (2015). Silver nanoparticles disrupt the regulation of steroidogenesis in fish ovarian cells. Aquatic Toxicology, 169, 143-151.

Dosumu, O. O., Akang, E. N., Idowu, O. K., & Adeyemi, G. J. (2021). Virgin Coconut (Cocos nucifera) Oil Attenuates Rotenone-Induced Toxicity inFruit Flies (Drosophila melanogaster). Journal of Basic and Social Pharmacy Research, 2(1), 26 - 37.

Dosumu, O., Duru, F., Osinubi, A., Oremosu, A., & Noronha, C. (2010). Influence of virgin coconut oil (VCNO) on oxidative stress, serum testosterone, and gonadotropic hormones (FSH, LH) in chronic ethanol ingestion. Agriculture and Biology Journal of North America, 6, 1126-1132.

Dringen, R. (2000). Metabolism and functions of glutathione in brain. Progress in Neurobiology, 62(6), 649-671.

Dröge, W., & Breitkreutz, R. (2000). Glutathione and immune function. Proceedings of the Nutrition Society, 59(4), 595-600.

Eleftherianos, I., More, K., Spivack, S., Paulin, E., Khojandi, A., & Shukla, S. (2014). Nitric oxide levels regulate the immune response of Drosophila melanogaster reference laboratory strains to bacterial infections. Infection and immunity, 82(10), 4169-4181.

Ellman, G. L., Courtney, K. D., Andres Jr, V., & Featherstone, R. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 7(2), 88-95.

Farombi, E. O., Abolaji, A. O., Farombi, T. H., Oropo, A. S., Owoje, O. A., & Awunah, M. T. (2018). Garcinia kola seed biflavonoid fraction (Kolaviron), increases longevity and attenuates rotenone-induced toxicity in Drosophila melanogaster. Pesticide biochemistry and physiology, 145, 39-45.

Fernando, W. M., Martins, I. J., Goozee, K., Brennan, C. S., Jayasena, V., & Martins, R. N. (2015). The role of dietary coconut for the prevention and treatment of Alzheimer's disease: potential mechanisms of action. British Journal of Nutrition, 114(1), 1-14.

Fu, L., Liu, K., Sun, M., Tian, C., Sun, R., Betanzos, C. M., ... Yang, J. (2017). Systematic and quantitative assessment of hydrogen peroxide reactivity with cysteines across human proteomes. Molecular & Cellular Proteomics, 16(10), 1815-1828.

Gökçal, E., Veysel Eren, G., Selvitop, R., Yildiz, G. B., & Talip, A. (2017). Motor and non-motor symptoms in Parkinson’s disease: effects on quality of life. Archives of Neuropsychiatry, 54(2), 143.

Grotto, D., Maria, L. S., Valentini, J., Paniz, C., Schmitt, G., Garcia, S. C., . . . Farina, M. (2009). Importance of the lipid peroxidation biomarkers and methodological aspects for malondialdehyde quantification. Quimica Nova, 32(1), 169-174.

Hirth, F., & Targets, N. D. (2010). Drosophila melanogaster in the study of human neurodegeneration. CNS & Neurological Disorders- Drug Targets 9(4), 504-523.

Hu, X., Fu, W., Yang, X., Mu, Y., Gu, W., & Zhang, M. (2019). Effects of cadmium on fecundity and defense ability of Drosophila melanogaster. Ecotoxicology and environmental safety, 171, 871-877.

Ilie, O. D., Ciobica, A., McKenna, J., Doroftei, B., & Mavroudis, I. (2020). Minireview on the relations between gut microflora and Parkinson’s disease: Further biochemical (oxidative stress), inflammatory, and neurological particularities. Oxidative medicine and cellular longevity, 2020.

Izaguirry, A. P., Soares, M. B., Vargas, L. M., Spiazzi, C. C., dos Santos Brum, D., Noremberg, S., . . . Santos, F. W. (2017). Blueberry (Vaccinium ashei Reade) extract ameliorates ovarian damage induced by subchronic cadmium exposure in mice: Potential δ‐ALA‐D involvement. Environmental Toxicology, 32(1), 188-196.

Jellinger, K. A. (2014). Neurobiology of Non-Motor Symptoms in Parkinson's Disease. Journal of Neurological Disorders & Stroke, 2, 1032.

Johnson, K. A., Conn, P. J., & Niswender, C. M. (2009). Glutamate receptors as therapeutic targets for Parkinson's disease. CNS & Neurological Disorders-Drug Targets (Formerly Current Drug Targets-CNS & Neurological Disorders), 8(6), 475-491.

Kangralkar, V., Patil, S. D., & Bandivadekar, R. (2010). Oxidative stress and diabetes: a review. International Journal of Pharmaceutical Applications, 1(1), 38-45.

Karatas, F., Karatepe, M., & Baysar, A. (2002). Determination of free malondialdehyde in human serum by high-performance liquid chromatography. Analytical Biochemistry, 311(1), 76-79.

Kumar, A., Goyal, K., Pal, M., Upadhyay, P., Gupta, S., Koul, V., & Konar, A. J. b. (2022). Preclinical efficacy of bacosides-lauric acid nano-herbal formulation in comparison to rivastigmine for treatment of Alzheimer’s disease.

Lee, F. K., Wong, A. K., Lee, Y. W., Wan, O. W., Edwin Chan, H., & Chung, K. K. (2009). The role of ubiquitin linkages on α‐synuclein induced toxicity in a Drosophila model of Parkinson’s disease. Journal of neurochemistry, 110(1), 208-219.

Lee, S., Bang, S. M., Lee, J. W., & Cho, K. S. (2014). Evaluation of traditional medicines for neurodegenerative diseases using Drosophila models. Evidence-Based Complementary and Alternative Medicine, 967462.

Lu, B., & Vogel, H. (2009). Drosophila models of neurodegenerative diseases. Annual Review of Pathology: Mechanisms of Disease, 4, 315-342.

Magerkurth, C., Schnitzer, R., & Braune, S. (2005). Symptoms of autonomic failure in Parkinson’s disease: prevalence and impact on daily life. Clinical Autonomic Research, 15(2), 76-82.

Maher, P., Lewerenz, J., Lozano, C., & Torres, J. (2008). A novel approach to enhancing cellular glutathione levels. Journal of Neurochemistry, 107(3), 690-700.

McIntosh, J., & Wilson, D. R. (2018). What is a serotonin and what does it do? Medical News Today.

Mizuno, H., Fujikake, N., Wada, K., & Nagai, Y. (2011). α-Synuclein transgenic Drosophila as a model of Parkinson's disease and related synucleinopathies. Parkinson’s Disease, 2011.

Mostoufi, S. L., & Singh, N. D. (2022). Diet-induced changes in titer support a discrete response of Wolbachia-associated plastic recombination in Drosophila melanogaster. G3, 12(1), jkab375.

Muñoz-Soriano, V., & Paricio, N. (2011). Drosophila models of Parkinson's disease: discovering relevant pathways and novel therapeutic strategies. Parkinson's Disease, 2011.

Nafar, F., Clarke, J., & Mearow, K. (2017). Coconut oil protects cortical neurons from amyloid beta toxicity by enhancing the signaling of cell survival pathways. Neurochemistry International, 105, 64-79.

Nandi, A., Yan, L.-J., Jana, C. K., & Das, N. (2019). Role of catalase in oxidative stress-and age-associated degenerative diseases. Oxidative medicine and cellular longevity, 2019.

Ordóñez, N. (2018). A global genetic diversity analysis of Fusarium oxysporum f. sp. cubense: the Panama disease pathogen of banana, in Ecuador. Plants, 9(9), 1133.

Pang, K.-L., Lumintang, J. N., & Chin, K.-Y. (2021). Thyroid-modulating activities of olive and its polyphenols: A systematic review. Nutrients, 13(2), 529.

Poewe, W., Seppi, K., Tanner, C. M., Halliday, G. M., Brundin, P., Volkmann, J., . . . Lang, A. E. (2017). Parkinson disease. Nature reviews Disease primers, 3(1), 1-21.

Ragonese, P., D’amelio, M., Salemi, G., Aridon, P., Gammino, M., Epifanio, A., . . . Savettieri, G. (2004). Risk of Parkinson's disease in women: effect of reproductive characteristics. Neurology, 62(11), 2010-2014.

Rao, S. V., Yenisetti, S. C., & Rajini, P. S. (2016). Evidence of neuroprotective effects of saffron and crocin in a Drosophila model of parkinsonism. Neurotoxicology, 52, 230-242.

Riederer, P., Berg, D., Casadei, N., Cheng, F., Classen, J., Dresel, C., . . . Reichmann, H. (2019). α-Synuclein in Parkinson’s disease: causal or bystander? Journal of neural transmission, 126(7), 815-840.

Russo, G. L., Spagnuolo, C., Russo, M., Tedesco, I., Moccia, S., & Cervellera, C. (2020). Mechanisms of aging and potential role of selected polyphenols in extending healthspan. Biochemical pharmacology, 173, 113719.

Sakakibara, R., Uchiyama, T., Yamamoto, T., Kishi, M., Ogawa, E., & Tateno, F. (2012). Sexual Problems in Parkinson’s Disease. Psychiatry of Parkinson's Disease, 27, 71-76.

Sedlak, J., & Lindsay, R. (1968). Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Analytical Biochemistry 25, 192-205.

Siddiqui, I. J., Pervaiz, N., & Abbasi, A. (2016). The Parkinson disease gene SNCA: evolutionary and structural insights with pathological implication. Scientific Reports, 6(1), 1-11.

Sule, W. F., Oyeyemi, M. O., & Akusu, M. O. (2007). Coconut milk-citrate as an extender for West African dwarf buck spermatozoa at room temperature. Biochemistry, 19(2).

Sun, H., Wu, W., Guo, J., Xiao, R., Jiang, F., Zheng, L., & Zhang, G. (2016). Effects of nickel exposure on testicular function, oxidative stress, and male reproductive dysfunction in Spodoptera litura Fabricius. Chemosphere, 148, 178-187.

Sun, M., & Zigman, S. (1978). An improved spectrophotometric assay for superoxide dismutase based on epinephrine autoxidation. Analytical biochemistry, 90(1), 81-89.

Suryani, S., Sariani, S., Earnestly, F., Marganof, M., Rahmawati, R., Sevindrajuta, S., . . . Fudholi, A. (2020). A comparative study of virgin coconut oil, coconut oil, and palm oil in terms of their active ingredients. Processes, 8(4), 402.

Sveinbjornsdottir, S. (2016).The clinical symptoms of Parkinson's disease.Journal of Neurochemistry. 139(1), 318–324.

Zhang, X., Lu, L., Liu, S., Ye, W., Wu, J., & Zhang, X. (2013). Acetylcholinesterase deficiency decreases apoptosis in dopaminergic neurons in the neurotoxin model of Parkinson's disease. The international journal of biochemistry & cell biology, 45(2), 265-272.

Zhuo, C., Zhu, X., Jiang, R., Ji, F., Su, Z., Xue, R. and Zhou, Y. (2017). Comparison for Efficacy and Tolerability among Ten Drugs for Treatment of Parkinson’s Disease: A Network Meta-Analysis. Scientific Reports. 7: 45865.